Artificial surface effect on red blood cells and platelets in laminar shear flow.
作者： TM Alkhamis , RL Beissinger , JR Chediak
DOI: 10.1182/BLOOD.V75.7.1568.1568
关键词:
摘要: Red blood cell (RBC) effects on platelet adhesion to a nonbiologic test surface (tetrafluoroethylene propylene copolymer) and aggregation during laminar shear flow for rates 5,680 s-1 (corresponding stress 200 dyne/cm2) were investigated. Results hemoglobin (Hb) adenosine diphosphate (ADP) release from RBCs, percent decrease of single platelets in the bulk, covered with obtained cone-and-plate (CP) viscometer samples whole blood, suspensions RBC ghosts platelet-rich plasma (PRP), RBCs either PRP or platelet-poor plasma. over rate range studied normal hematocrit indicated that low-stress shearing led ADP Hb intact RBCs; shear-induced was about twice platelets, total released, released contributed six times reduction bulk adhesion, ie, coverage platelets. various hematocrits showed above threshold 25% 35% (suspended PRP) had greater contribution release, than themselves. Single suspended correlated level not stress.
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bloodjournal.org参考文章(22)
R L Beissinger, T M Alkhamis, J R Chediak, Red blood cell effect on platelet adhesion and aggregation in low-stress shear flow. Myth or fact? Asaio Journal. ,vol. 34, pp. 868- 873 ,(1988)
R C Reimers, R K Baker, M W Moritz, S P Sutera, J H Joist, Role of cytoplasmic and releasable ADP in platelet aggregation induced by laminar shear stress Journal of Laboratory and Clinical Medicine. ,vol. 101, pp. 537- 544 ,(1983)
WILLIAM H. CROSBY, FRANK W. FURTH, CAROL E. THIBEAULT, A modification of the benzidine method for measurement of hemoglobin in plasma and urine. Blood. ,vol. 11, pp. 380- 383 ,(1956) , 10.1182/BLOOD.V11.4.380.380
P. Jououanel, C. Motta, J. Delattre, B. Dastugue, A rapid and sensitive fluorometric assay of serum phospholipid Clinica Chimica Acta. ,vol. 105, pp. 173- 181 ,(1980) , 10.1016/0009-8981(80)90459-3
V. Turitto, H. Weiss, Red blood cells: their dual role in thrombus formation Science. ,vol. 207, pp. 541- 543 ,(1980) , 10.1126/SCIENCE.7352265
G. V. R. BORN, Aggregation of blood platelets by adenosine diphosphate and its reversal. Nature. ,vol. 194, pp. 927- 929 ,(1962) , 10.1038/194927B0
R. L. Beissinger, M. C. Williams, A dual mechanism for low‐stress hemolysis in laminar blood flow Aiche Journal. ,vol. 30, pp. 569- 577 ,(1984) , 10.1002/AIC.690300407
James T. Willerson, L. David Hillis, Michael Winniford, L. Maximilian Buja, Speculation regarding mechanisms responsible for acute ischemic heart disease syndromes. Journal of the American College of Cardiology. ,vol. 8, pp. 245- 250 ,(1986) , 10.1016/S0735-1097(86)80121-8
J. Molnar, L. Lorand, Studies on apyrases Archives of Biochemistry and Biophysics. ,vol. 93, pp. 353- 363 ,(1961) , 10.1016/0003-9861(61)90278-8
A. Andersen, E. Myhre, K. Rasmussen, Serum lactic dehydrogenase activity in patients with prosthetic heart valves: a parameter of intravascular hemolysis. American Heart Journal. ,vol. 80, pp. 463- 468 ,(1970) , 10.1016/0002-8703(70)90192-4