Hypericin and photodynamic therapy decreases human pancreatic cancer in vitro and in vivo

摘要: Background. The treatment of pancreatic cancer has remained dismal despite advances in medical and surgical care. Recent preclinical data have revealed that hypericin, a photochemical dye, is activated by green light generates toxic radical species tumors. We hypothesized interstitial hypericin laser phototherapy would decrease growth. Methods. MiaPaCa-2 PANC-1 cells were grown tissue culture. In vitro experiments performed with addition 10 μg hypericin/500,000 cells. Cells incubated for 2 h. then exposed to KTP532 1 min at 0.6 W using cylindrical diffuser tip. Cell growth was measured MTT assay 24 h after treatment, N = 12. implanted subcutaneously orthotopically pancreas nude mice. After 5 weeks, both tumors injected 100 followed insertion tip into the tumor center. Mice received 200J KTP 1.0 two sites. Tumors before 4 weeks treatment. Results. Both vivo mice showed significant cancer. Pancreatic cell suppressed 66.1 ± 0.2%, n 12, P < 0.01, ANOVA. Subcutaneous shoulder 91.2 2.3%, 0.001, 42.2 8.1%, 0.05, compared pretreatment sizes. Data expressed as percentage reduction vs paired controls pre-photodynamic therapy experiments. Paired Student's t tests sizes. Conclusion. results growth. Laser or dye alone had no effect, indicating intratumor may prove useful unresectable