Metabarcoding of Soil Fungal Communities Associated with Alpine Field-Grown Saffron (Crocus sativus L.) Inoculated with AM Fungi.

摘要: Soil fungi strongly influence ecosystem structure and functioning, playing a key role in many ecological services as decomposers, plant mutualists pathogens. Arbuscular mycorrhizal (AMF) establish mutualistic symbiotic associations with roots act biofertilizers by enhancing nutrients water uptake. Information about the AMF association Crocus sativus L. (saffron) their impact on crop performances spice quality has been increasing recent years. Instead, there is still little data biodiversity of soil microbial communities associated this Alpine environments. The aims study were to investigate fungal two experimental sites cultivated saffron, rank relative inocula, applied single species (R = Rhizophagus intraradices, C. Walker & A. Schusler) or mixture (M R. intraradices Funneliformis mosseae, Schusler), resident which might be influenced diversity composition. We used Illumina MiSeq metabarcoding nuclear ribosomal ITS2 region characterize cultivation fields, located municipalities Saint Christophe (SC) Morgex (MG), (Aosta Valley, Italy), treated not inocula sampled for consecutive years (Y1; Y2). Data analyses consistently indicated that Basidiomycota particularly abundant both sampling (Y1 Significant differences distribution taxa assemblages at phylum class levels between also found. main compositional consisted significant abundance changes OTUs belonging Dothideomycetes Leotiomycetes (Ascomycota), Agaricomycetes Tremellomycetes (Basidiomycota), Mortierellomycetes Mucoromycetes. Further concerned OTUs, other classes, significantly represented only first second year sampling. Concerning Glomeromycota, most genus was Claroideoglomus always detected Other genera such Funneliformis, Septoglomus Microdominikia, retrieved MG site. Results highlighted neither nor inoculation impacted saffron-field communities; instead, had appreciable communities.