Erythropoietin (EPO) increases myelin gene expression in CG4 oligodendrocyte cells through the classical EPO receptor

摘要: Erythropoietin (EPO) has protective effects in neurodegenerative and neuroinflammatory diseases, including animal models of multiple sclerosis, where EPO decreases disease severity. also promotes neurogenesis is toxic demyelination. In this study, we asked whether could promote neurorepair by inducing remyelination. addition, investigated the effect be mediated classical erythropoietic receptor (EPOR), since it still questioned if EPOR functional nonhematopoietic cells. Using CG4 cells, a line rat oligodendrocyte precursor found that increases expression myelin genes (myelin glycoprotein (MOG) basic protein (MBP)). had no wild-type which do not express EPOR, whereas increased MOG MBP cells engineered to overexpress (CG4-EPOR). This was reflected marked increase levels, as detected Western blot. these induced 10-fold early growth response gene 2 (Egr2), required for peripheral myelination. However, Egr2 silencing with siRNA did reverse EPO, indicating acts through other pathways. conclusion, induces oligodendrocytes requires presence EPOR. study demonstrates can mediate neuroreparative effects.