Differential protection of normal and malignant human myeloid progenitors (CFU-GM) from Ara-C toxicity using cycloheximide

摘要: Cycloheximide, a reversible protein synthesis inhibitor, is thought to block DNA replication in normal cells by preventing of labile protein. In animal systems, cycloheximide protects from cytotoxic S-phase specific agents, such as cytosine arabinoside (Ara-C). Malignant appear not be susceptible cycloheximide-induced cycle arrest and, subsequently, may protected Ara-C cytotoxicity. The effect on granulocyte/macrophage progenitors (CFU-GM) after vitro exposure was examined using human bone marrow, malignant patients with chronic myelogenous leukemia (CML), and clonogenic the acute nonlymphocytic cell lines HL-60 KG-1. Mononuclear or were incubated for one hour cycloheximide, followed addition, three 17 hours, before being plated methylcellulose culture system. CFU-GM survival significantly increase if treated exposure. Similar pretreatment CML KG-1 failed protect Ara-C-induced