Development of a 5'-nuclease PCR assay for detecting Shiga toxin-producing Escherichia coli O145 based on the identification of an 'O-island 29' homologue.
作者： S. Perelle , F. Dilasser , J. Grout , P. Fach
DOI: 10.1046/J.1365-2672.2003.01872.X
关键词:
摘要: Aims: A DNA sequence, from Escherichia coli STEC O145, homologous to O-island 29 O157 is described, together with a real-time PCR assay for detecting it. Methods and Results: sequencing were used identify the ‘O-island 29’ sequence O145 (strain VTH34). The divergence between allowed 5′-nuclease be developed. Conclusions: characterization of novel locus in has specific serogroup designed. Significance Impact Study: These findings increase number assays available as alternatives classical O-serotyping E. coli.
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