Identification and biodegradation potential of tropical aerobic hydrocarbon-degrading microorganisms.

摘要: Screening of aerobic culturable hydrocarbon (HC)-degrading microorganisms isolated from petroleum-polluted soils and cyanobacterial mats Indonesia resulted in the collection 33 distinct species. Eight bacteria, 21 fungi 4 yeasts were identified to specific level by molecular phenotypic techniques. Bacterial strains belonged genera Gordonia, Brevibacterium, Aeromicrobium, Dietzia, Burkholderia Mycobacterium. Four species are new not yet described. Fungi Aspergillus, Penicillium, Fusarium, Amorphoteca, Neosartorya, Paecilomyces, Talaromyces Graphium. Yeasts Candida, Yarrowia Pichia. All cultivated axenically synthetic liquid media with crude oil as sole carbon energy source. After incubation, detailed chemical composition residual was studied gravimetric gas-chromatographic Thirteen parameters for assessing biodegradation potential defined computed each strain. Maximum degradation observed on saturated HCs (n- isoalkanes, isoprenoids), whereas aromatic HC lower related structural molecules. A principal components analysis (PCA) permitted grouping classifying a function their degradative capacities. It shown that most active produced polar metabolites which accumulated resins asphaltene fractions. These fractions highly resistant microbial metabolism. No taxonomic trend could be between phyla terms activity.