Urine analysis of 3,4-methylenedioxypyrovalerone in opioid-dependent patients by gas chromatography-mass spectrometry.

摘要: A gas chromatography-mass spectrometry (GCMS) procedure was developed for the quantitative analysis of new designer drug methylenedioxypyrovalerone (MDPV) in urine together with common stimulants amphetamine, methamphetamine, and methylenedioxymethamphetamine (MDMA). The involved electron ionization (EI) GCMS selected ion monitoring (SIM) mode after liquid-liquid extraction toluene derivatization heptafluorobutyric acid anhydride. All MDPV findings were confirmed by positive chemical SIM mode. Positive ionization-GCMS allowed protonated molecule M+H+ m/z 276 to be used as a target 3 abundant fragments qualifier ions. By ionization-GCMS, limit quantification (LOQ) 0.02 mg/L; MDMA, LOQ 0.05 mg/L. method applied samples from opioid-dependent patients undergoing opioid substitution treatment. Nine 34 (26%) analyzed procedure. obtained 2 female 7 male mean age 31 years. median (range) concentration 0.16 mg/L (0.04-3.9 mg/L) based on which numeric value obtained, whereas below but above detection samples. revealed amphetamine approximately 40% cases, there no statistical difference between MDPV-positive MDPV-negative groups. Urine concentrations average 10 times higher than those MDPV. mainly substitute judging laboratory this study information our patients.