Harvesting and subculturing cells growing on denatured-collagen coated microcarriers (Cytodex 3).

摘要: Selecting correct procedures and conditions for harvesting cells was essential successful subcultivation of when using the microcarrier culture technique. The proteolytic enzymes trypsin, Dispase collagenase were compared with respect to yield viability Vero harvested from Cytodex 3 microcarriers. Treatment or trypsin resulted in similar viabilities but recovery after treatment improved. advantage improved plating efficiency subculturing new cultures. Pre-washing confluent microcarriers EDTA (0.02% w/v) collagenase. stage cycle did not influence relative viability. In contrast, significant differences found grown presence different batches foetal calf serum. results confirm that choice enzyme depends upon cell type purpose culture.