Rapid identification of Paracoccidioides lutzii and P. Brasiliensis using Fourier Transform Infrared spectroscopy

摘要: Abstract The inhalation of the fungus Paracoccidioides brasiliensis (P. brasiliensis, Pb03 and Pb18) causes Parcoccidioidomycosis. Initially, only P. brasiliensis was thought to be responsible for it. Later researchers found out that another pathogen: lutzii (P. lutzii, Pb01) may also cause disease. Clinical manifestation treatment differ based on causative agent, making it important identify them correctly. Therefore, this study focusses distinguishing from P. lutzii using Fourier Transform Infrared (FTIR) spectroscopy - a rapid, highly specific sensitive technique can biochemical composition with minimal sample preparation. We acquired thirty spectra each pure cultures P. lutzii, P. brasiliensis. FTIR showed difference in composition, major differences being polysaccharide proteins vibrations. Principal Component Linear Discriminant Analysis (PC-LDA) could distinguish species. nearly 10 per triplicates, resulting around 30 group. analysis indicates difference. Multivariate is spectral chosen regions where we take biological or vibration information. PC-LDA Pb01 (P. lutzii) 96% efficiency Pb03/Pb18 (P.brasiliensis) 100% efficiency. Thus, contribute clinical diagnosis prevalent Latin American