Loop-mediated isothermal PCR (LAMP) for the diagnosis of falciparum malaria.
作者： Daniel H. Paris , Sue J. Lee , Abul M. Faiz , Mahtabuddin Hasan , Mallika Imwong
DOI: 10.4269/AJTMH.2007.77.972
关键词:
摘要: A recently described loop-mediated isothermal polymerase chain reaction (LAMP) for molecular detection of Plasmodium falciparum was compared with microscopy, PfHRP2-based rapid diagnostic test (RDT), and nested (PCR) as the "gold standard" in 115 Bangladeshi in-patients fever. DNA extraction LAMP conducted by conventional methods or simple heating sample; results were either assessed visually gel electrophoresis. Conventional followed electrophoresis had highest agreement reference method (81.7%, kappa = 0.64), a sensitivity (95% CI) 76.1% (68.3-83.9%), comparable to RDT but specificity 89.6% (84.0-95.2%) 100% microscopy. heat treatment deteriorated unacceptable levels. enables diagnosis malaria settings limited technical resources will need further optimization. The are contrast higher accuracy reported an earlier study comparing non-validated PCR method.
tropmedres.ac参考文章(19)
Yasuyoshi Mori, Kentaro Nagamine, Norihiro Tomita, Tsugunori Notomi, Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochemical and Biophysical Research Communications. ,vol. 289, pp. 150- 154 ,(2001) , 10.1006/BBRC.2001.5921
Georges Snounou, Suganya Viriyakosol, Xin Ping Zhu, William Jarra, Lucilia Pinheiro, Virgilio E. do Rosario, Sodsri Thaithong, K.Neil Brown, High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction Molecular and Biochemical Parasitology. ,vol. 61, pp. 315- 320 ,(1993) , 10.1016/0166-6851(93)90077-B
E. Tjitra, S. Suprianto, J. McBroom, B. J. Currie, N. M. Anstey, Persistent ICT malaria P.f/P.v panmalarial and HRP2 antigen reactivity after treatment of Plasmodium falciparum malaria is associated with gametocytemia and results in false-positive diagnoses of Plasmodium vivax in convalescence. Journal of Clinical Microbiology. ,vol. 39, pp. 1025- 1031 ,(2001) , 10.1128/JCM.39.3.1025-1031.2001
L M Milne, M S Kyi, P L Chiodini, D C Warhurst, Accuracy of routine laboratory diagnosis of malaria in the United Kingdom. Journal of Clinical Pathology. ,vol. 47, pp. 740- 742 ,(1994) , 10.1136/JCP.47.8.740
Mayfong Mayxay, Sasithon Pukrittayakamee, Kesinee Chotivanich, Sornchai Looareesuwan, Nicholas J. White, Persistence of Plasmodium falciparum HRP-2 in successfully treated acute falciparum malaria. Transactions of The Royal Society of Tropical Medicine and Hygiene. ,vol. 95, pp. 179- 182 ,(2001) , 10.1016/S0035-9203(01)90156-7
Masanori Hagiwara, Hajime Sasaki, Koma Matsuo, Mariko Honda, Masaaki Kawase, Hidemi Nakagawa, Loop-mediated isothermal amplification method for detection of human papillomavirus type 6, 11, 16, and 18. Journal of Medical Virology. ,vol. 79, pp. 605- 615 ,(2007) , 10.1002/JMV.20858
D C Warhurst, J E Williams, ACP Broadsheet no 148. July 1996. Laboratory diagnosis of malaria. Journal of Clinical Pathology. ,vol. 49, pp. 533- 538 ,(1996) , 10.1136/JCP.49.7.533
N.J. White, K. Silamut, Rapid diagnosis of malaria. The Lancet. ,vol. 333, pp. 435- 435 ,(1989) , 10.1016/S0140-6736(89)90025-1
Stephane Proux, Lily Hkirijareon, Chatee Ngamngonkiri, Stephen McConnell, Francois Nosten, Paracheck-Pf: a new, inexpensive and reliable rapid test for P. falciparum malaria. Tropical Medicine & International Health. ,vol. 6, pp. 99- 101 ,(2001) , 10.1046/J.1365-3156.2001.00694.X
T. Notomi, Loop-mediated isothermal amplification of DNA Nucleic Acids Research. ,vol. 28, ,(2000) , 10.1093/NAR/28.12.E63