Iron overload increases osteoclastogenesis and aggravates the effects of ovariectomy on bone mass

摘要: Postmenopausal osteoporosis is a metabolic disease associated with estrogen deficiency. The results of numerous studies have revealed the positive correlation between iron accumulation and postmenopausal osteoporotic status. Although previous indicated that or alone an effect on bone metabolism, their combined effects are not well defined. Using in vivo mouse model, we found mass was minimally affected by excess presence estrogen. Once source removed (ovariectomy), significantly decreased mass. These were accompanied fluctuations level oxidative stress. To determine whether these related to formation resorption, primary osteoblasts (OBs), RAW264.7 cells, bone-marrow-derived macrophages used for vitro experiments. We did inhibit activity OBs. However, had little this inhibition. In contrast, promoted osteoclast differentiation through production reactive oxygen species. Estrogen, powerful scavenger, suppressed osteoclasts. Our data provided direct evidence only absence inhibitory iron-induced osteopenia particularly relevant resorption rather than formation.