Resistance of mice to experimental leishmaniasis is associated with more rapid appearance of mature macrophages in vitro and in vivo.

摘要: Resistance to murine leishmaniasis has been related the propagation of specific Th cell subsets (Th1 and Th2). This study shows that there are differences between resistant susceptible mice in initial myelomonocytic infiltrate, which precede T response. After subcutaneous injection 2 x 10(7) Leishmania major into footpads C57Bl/6 BALB/c we performed immunohistochemical studies on infiltrate. Two days after infection percentage more mature, F4/80-positive macrophages lesion increased faster (63%) than (29%). The same strain-specific were observed corresponding strains athymic (57.2% nu/nu; 33.6% nu/nu), thus excluding a cell-controlled phenomenon. 1 wk infiltrate began reveal significantly cells containing MRP14, is expressed by granulocytes less mature monocytes but not macrophages. No found strains, suggesting at this point organization falls under control protective cells. In bone marrow cultures mice, was also increasing mice. Increased expression F4/80 Ag associated with higher leishmanicidal activity MRP14-positive other hand rarely infected parasites. We suggest 1) earlier appearance lesions could influence either Th1 or Th2 reduction parasite load differential secretion decisive cytokines 2) diffuse accumulation inflammatory facilitates spread disease.