355 COMPOUND FIELD POTENTIALS VARY WITH INNERVATION OF THE PREGNANT RAT CERVIX

摘要: Previously we have demonstrated that innervation of rodent cervix is increased before birth, a process also described in humans. The objective this study was to determine the physiological contribution neural input from vagus, hypogastric, and pelvic nerves. Pregnant (D19-D21), post-partum, non-pregnant Sprague-Dawley rats were anesthetized with Nembutal (40mg/kg), injected intraperitoneally, paralyzed Gallamine triethiodide (2mg/kg) and/or Pancuronium bromide (2mg/kg). Animals maintained on artificial respiration then dissected using midline incision expose abdominal viscera. vagus (subdiaphram), hypogastric nerves isolated stimulated bipolar electrode. Signals recorded concentric electrode (Fred Haer Inc-CBEEJ75) connected an ETH-250 Bioamplifier (CB Sciences). Field potentials analyzed MacLab 4e Chart 3.6. Nerve conduction velocities range slow unmyelinated C-afferent pain fibers. current required reach threshold was, general, larger post partum groups than late term pregnant rats. In non-pregnant, post-partum respectively, 30 mA, 5 mA for 20-, 18-, 2 30-, 0.1 These observations raise possibility reduced thresholds cervical field reflect birth. This increase may contribute ripening essential parturition. Supported by generous grant form John Mace Pediatric Grant Dean School Medicine.