Characterization of cyclin-dependent kinases and their expression in developing maize endosperm

摘要: During maize endosperm development, cells switch from mitotic into endoreduplication cell cycles, concomitant with the differentiation of starchy cells. The identity and role cyclins cyclin-dependent kinases (CDKs) that control these cycles are poorly understood. I identified characterized D(CYCD2;1 CYCD5;1) A1-types (CYCA1;3) expressed in endosperm. CYCA1;3 RNA decreased sharply as transitioned to stage, whereas CYCD2;1 CYCD5;1 transcripts declined gradually. Polyclonal antibodies against CYCA1;3, CYCD5;1, a B-type homologue, CYCB1;3, were used characterize cyclin protein accumulation, associated CDK activity, spatial localization Except for levels nearly constant or slightly throughout development. activity was most abundant 7 days after pollination (DAP), while peaked at 11 DAP. appeared be cytoplasm non-endoreduplicating CYCB1;3 found endosperm, mostly