Tudor domain proteins in protozoan parasites and characterization of Plasmodium falciparum tudor staphylococcal nuclease.
作者： Manzar J. Hossain , Reshma Korde , Shivani Singh , Asif Mohmmed , P.V.N. Dasaradhi
DOI: 10.1016/J.IJPARA.2007.09.018
关键词:
摘要: RNA-binding proteins play key roles in post-transcriptional regulation of gene expression. In eukaryotic cells, a multitude with several domains/motifs have been described. Here, we show the existence two Tudor domain containing proteins, survival motor neuron (SMN)-like protein and Staphylococcus aureus nuclease homologue referred to as TSN, Plasmodium other protozoan parasites. Activity analysis shows that falciparum TSN (PfTSN) possesses activity is domain. A specific inhibitor micrococcal nucleases, 3',5'-deoxythymidine bisphosphate (pdTp) inhibits well activities protein. PfTSN predominant nuclear localization. Treatment P. pdTp, inhibited vitro growth both chloroquine-sensitive chloroquine-resistant strains falciparum, while four fold concentration pdTp did not any significant effect on mammalian cell line, Huh-7D12. Altogether, these results suggest an essential enzyme parasite's life cycle.
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