Microfluidic assay for simultaneous culture of multiple cell types on surfaces or within hydrogels
作者： Yoojin Shin , Sewoon Han , Jessie S Jeon , Kyoko Yamamoto , Ioannis K Zervantonakis
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摘要: This protocol describes a simple but robust microfluidic assay combining three-dimensional (3D) and two-dimensional (2D) cell culture. The microfluidic platform comprises hydrogel-incorporating chambers between surface-accessible microchannels. By using this platform, well-defined biochemical and biophysical stimuli can be applied to multiple cell types interacting over distances of< 1 mm, thereby replicating many aspects of the in vivo microenvironment. Capabilities exist for time-dependent manipulation of flow and …
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sci-hub.se 参考文章(28)
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