Proteome of the Triatomine Digestive Tract: From Catalytic to Immune Pathways; Focusing on Annexin Expression.

摘要: Rhodnius prolixus, Panstrongylus megistus, Triatoma infestans, and Dipetalogaster maxima are all triatomines potential vectors of the protozoan Trypanosoma cruzi responsible for human Chagas' disease. Considering that T. cruzi's cycle occurs inside triatomine digestive tract (TDT), analysis TDT protein profile is an essential step to understand physiology during infection. To characterize D. maxima, P. R. a shotgun liquid chromatography-tandem mass spectrometry (LC-MS/MS) approach was applied in this report. Most proteins were found be closely related metabolic pathways such as gluconeogenesis/glycolysis, citrate cycle, fatty acid metabolism, oxidative phosphorylation, but also immune system. We annotated new proteome contribution gathering it with those previously published accordance Gene Ontology KEGG. Enzymes classified terms class, acceptor, function, while from system by reference humoral response, cell regulation, Toll, IMD, JNK, Jak-STAT, MAPK, available Insect Innate Immunity Database (IIID). These further subclassified recognition, signaling, coagulation, melanization none. Finally, phylogenetic affinities gene expression annexins investigated understanding their role protection homeostasis intestinal epithelial cells against inflammation.