Nanoparticle Uptake in Plants: Gold Nanomaterial Localized in Roots of Arabidopsis thaliana by X-ray Computed Nanotomography and Hyperspectral Imaging.

作者: Astrid Avellan , Fabienne Schwab , Armand Masion , Perrine Chaurand , Daniel Borschneck

DOI: 10.1021/ACS.EST.7B01133

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摘要: Terrestrial plants can internalize and translocate nanoparticles (NPs). However, direct evidence for the processes driving NP uptake distribution in is scarce at cellular level. Here, NP-root interactions were investigated after 10 days of exposure Arabidopsis thaliana to mg·L–1 negatively or positively charged gold NPs (∼12 nm) gels. Two complementary imaging tools used: X-ray computed nanotomography (nano-CT) enhanced dark-field microscopy combined with hyperspectral (DF-HSI). The use these emerging techniques improved our ability detect visualize plant tissue: by spectral confirmation via DF-HSI, three dimensions nano-CT. resulting provides that detaching border-like cells (i.e., sheets border from root) associated mucilage accumulate trap irrespective particle charge. On contrary, on root cap behaved a charge-specific fashion: positive...

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