miR-101-3p/Rap1b signal pathway plays a key role in osteoclast differentiation after treatment with bisphosphonates
作者： Jie Li , You Li , Shengjie Wang , Hui Che , Jun Wu
DOI: 10.5483/BMBREP.2019.52.9.076
关键词:
摘要: Bisphosphonates are the mainstay of therapy worldwide for osteoporosis. However, bisphosphonates also have limitations. The objective this study was to determine role miR-101-3p/Rap1b signal pathway in osteoclast differentiation after treatment with bisphosphonates. Our results revealed that miR-101-3p an important regulator treated-osteoclasts. When down-regulated bone marrow-derived macrophage-like cells (BMMs), development mature osteoclasts promoted, and vice versa. alendronate decreased multinucleated cell number regardless whether knocked down or over-expressed. TRAP activity assay confirmed above results. Luciferase indicated a negative Rap1b. Western blot analysis protein expression level Rap1b BMMs transfected OV-miR-101-3p lower than empty vector. overexpression increased TRAP-positive cells, while inhibition numbers. In vivo data showed inhibited ovariectomized mice overexpressed blocked differentiation. Taken together, our demonstrate plays key [BMB Reports 2019; 52(9): 572-576].
bmbreports.org参考文章(24)
Michael Kohl, Sebastian Wiese, Bettina Warscheid, Cytoscape: software for visualization and analysis of biological networks. Methods of Molecular Biology. ,vol. 696, pp. 291- 303 ,(2011) , 10.1007/978-1-60761-987-1_18
Chenyang Zhao, Weijia Sun, Pengfei Zhang, Shukuan Ling, Yuheng Li, Dingsheng Zhao, Jiang Peng, Aiyuan Wang, Qi Li, Jinping Song, Cheng Wang, Xiaolong Xu, Zi Xu, Guohui Zhong, Bingxing Han, Yan-Zhong Chang, Yingxian Li, miR-214 promotes osteoclastogenesis by targeting Pten/PI3k/Akt pathway RNA Biology. ,vol. 12, pp. 343- 353 ,(2015) , 10.1080/15476286.2015.1017205
Yanrui Sheng, Shijia Ding, Ke Chen, Juan Chen, Sen Wang, Chengcheng Zou, Jingnan Zhang, Yiyi Cao, Ailong Huang, Hua Tang, Functional analysis of miR-101-3p and Rap1b involved in hepatitis B virus-related hepatocellular carcinoma pathogenesis Biochemistry and Cell Biology. ,vol. 92, pp. 152- 162 ,(2014) , 10.1139/BCB-2013-0128
Ke Ke, Ok-Joo Sul, Monisha Rajasekaran, Hye-Seon Choi, MicroRNA-183 increases osteoclastogenesis by repressing heme oxygenase-1 Bone. ,vol. 81, pp. 237- 246 ,(2015) , 10.1016/J.BONE.2015.07.006
Gun-Il Im, Sheeraz A Qureshi, Jennifer Kenney, Harry E Rubash, Arun S Shanbhag, Osteoblast proliferation and maturation by bisphosphonates. Biomaterials. ,vol. 25, pp. 4105- 4115 ,(2004) , 10.1016/J.BIOMATERIALS.2003.11.024
Tony Yuen, Agnes Stachnik, Jameel Iqbal, Miriam Sgobba, Yogesh Gupta, Ping Lu, Graziana Colaianni, Yaoting Ji, Ling-Ling Zhu, Se-Min Kim, Jianhua Li, Peng Liu, Sudeh Izadmehr, Jaya Sangodkar, Jack Bailey, Yathin Latif, Shiraz Mujtaba, Solomon Epstein, Terry F. Davies, Zhuan Bian, Alberta Zallone, Aneel K. Aggarwal, Shozeb Haider, Maria I. New, Li Sun, Goutham Narla, Mone Zaidi, Bisphosphonates inactivate human EGFRs to exert antitumor actions Proceedings of the National Academy of Sciences of the United States of America. ,vol. 111, pp. 17989- 17994 ,(2014) , 10.1073/PNAS.1421410111
O. Johnell, J. A. Kanis, An estimate of the worldwide prevalence and disability associated with osteoporotic fractures. Osteoporosis International. ,vol. 17, pp. 1726- 1733 ,(2006) , 10.1007/S00198-006-0172-4
JIAN GONG, YI CHU, MEILI XU, JIRONG HUO, LIANG LV, Esophageal squamous cell carcinoma cell proliferation induced by exposure to low concentration of cigarette smoke extract is mediated via targeting miR-101-3p/COX-2 pathway Oncology Reports. ,vol. 35, pp. 463- 471 ,(2016) , 10.3892/OR.2015.4379
W. Zou, T. Izawa, T. Zhu, J. Chappel, K. Otero, S. J. Monkley, D. R. Critchley, B. G. Petrich, A. Morozov, M. H. Ginsberg, S. L. Teitelbaum, Talin1 and Rap1 are critical for osteoclast function. Molecular and Cellular Biology. ,vol. 33, pp. 830- 844 ,(2013) , 10.1128/MCB.00790-12
A. Subramanian, P. Tamayo, V. K. Mootha, S. Mukherjee, B. L. Ebert, M. A. Gillette, A. Paulovich, S. L. Pomeroy, T. R. Golub, E. S. Lander, J. P. Mesirov, Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profiles Proceedings of the National Academy of Sciences of the United States of America. ,vol. 102, pp. 15545- 15550 ,(2005) , 10.1073/PNAS.0506580102