The use of liquid polyacrylamide in electrophoresis: II. Relationship between gel viscosity and molecular sieving
作者： Hans-Joachim Bode
DOI: 10.1016/0003-2697(77)90045-8
关键词:
摘要: Abstract The effectiveness of molecular size discrimination in liquid polyacrylamide-agar mixed-gel electrophoresis is shown to depend on the polymer chain length. This relationship seems be mediated by intrinsic viscosity polymer. Possible implications for cross-linked gels are discussed.
elsevier.com
sci-hub.se 参考文章(31)
Electrophoretic studies on dyneins from Tetrahymena cilia. Journal of Biochemistry. ,vol. 76, pp. 991- 999 ,(1974) , 10.1093/OXFORDJOURNALS.JBCHEM.A130667
Joseph Gazith, Sylvia Himmelfarb, William F. Harrington, Studies on the Subunit Structure of Myosin Journal of Biological Chemistry. ,vol. 245, pp. 15- 22 ,(1970) , 10.1016/S0021-9258(18)63416-7
A K Dunker, R R Rueckert, Observations on Molecular Weight Determinations on Polyacrylamide Gel Journal of Biological Chemistry. ,vol. 244, pp. 5074- 5080 ,(1969) , 10.1016/S0021-9258(18)94310-3
L C Gershman, A Stracher, P Dreizen, Subunit Structure of Myosin III. A PROPOSED MODEL FOR RABBIT SKELETAL MYOSIN Journal of Biological Chemistry. ,vol. 244, pp. 2726- 2736 ,(1969) , 10.1016/S0021-9258(18)83456-1
David M. Neville, Molecular Weight Determination of Protein-Dodecyl Sulfate Complexes by Gel Electrophoresis in a Discontinuous Buffer System Journal of Biological Chemistry. ,vol. 246, pp. 6328- 6334 ,(1971) , 10.1016/S0021-9258(18)61792-2
Hans-Joachim Bode, The use of liquid polyacrylamide in electrophoresis: I. Mixed gels composed of agar-agar and liquid polyacrylamide Analytical Biochemistry. ,vol. 83, pp. 204- 210 ,(1977) , 10.1016/0003-2697(77)90527-9
K Weber, M Osborn, The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis Journal of Biological Chemistry. ,vol. 244, pp. 4406- 4412 ,(1969) , 10.1016/S0021-9258(18)94333-4
U E Loening, The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophoresis. The effects of changes in conformation Biochemical Journal. ,vol. 113, pp. 131- 138 ,(1969) , 10.1042/BJ1130131
Issei MABUCHI, A myosin-like protein in the cortical layer of cleaving starfish eggs. Journal of Biochemistry. ,vol. 76, pp. 47- 55 ,(1974) , 10.1093/OXFORDJOURNALS.JBCHEM.A130558
Neva Kingsbury, C.J. Masters, On the determination of component molecular weights in complex protein mixtures by means of disc electrophoresis. Analytical Biochemistry. ,vol. 36, pp. 144- 158 ,(1970) , 10.1016/0003-2697(70)90342-8