Microsequence analysis of peptides and proteins
作者： John E. Shively , Ursino Del Valle , Russell Blacher , David Hawke , Warren P. Levy
DOI: 10.1016/0003-2697(82)90522-X
关键词:
摘要: Abstract The sequence of the tryptic peptides three major species human leukocyte interferon was determined by microsequencing procedures. were aligned comparison with amino acid sequences predicted DNA recombinants containing interferon-coding inserts. In addition, extended NH 2 -terminal two interferons produced in Escherichia coli recombinant methodology are also reported. This report demonstrates application to low nanomole and subnanomole amounts proteins biological interest.
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