A new spectrophotometric assay for protein in cell extracts
作者： Vernon F. Kalb , Robert W. Bernlohr
DOI: 10.1016/0003-2697(77)90173-7
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摘要: Abstract Protein concentrations in cell-free extracts can be determined quickly and accurately using the measured absorbances at 230 260 nm. The choice of these two wavelengths, rather than those (280 nm) used by Warburg Christian [(1942) Biochem. Z.310, 384–421] , decreases interference nucleic acids results sensitivity equivalent to that method Lowry, Rosebrough, Farr, Randall [(1951) J. Biol. Chem.193, 265–275] . is less dependent on changes amino acid composition protein(s) being 280 method. Using buffered solutions crystalline bovine serum albumin yeast RNA as standards, we derived following equation: concentration (μg/ml) = 183 A230 − 75.8 A260, where A260 are
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