A colorimetric method for the determination of submicrogram quantities of protein
DOI: 10.1016/0003-2697(77)90011-2
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摘要: Abstract A simple procedure for the measurement of submicrogram quantities protein is described which can be used without interference from most common reagents. Protein-containing solutions are spotted on glass fiber filters, washed with trichloroacetic acid, and stained Coomassie blue. The filters destained, protein-bound colorant eluted measured in a spectrophotometer at 590 nm. response linear to 5 μg per filter, as little 0.1 filter accurately determined.
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