Properties and Applications of Monoclonal Antibodies Directed against Determinants of the Thy-1 Locus

摘要: Fusion of cells the mouse myeloma line, P3/X63-Ag8 with spleen from AKR/J mice immunized against C3H thymocytes or (BALB/c × BALB.K)F 1 gave rise to hybrid cell lines that continuously secrete antibodies specific for Thy-1.2 and Thy-1.1 antigens, respectively. Monoclonal four such were analyzed in detail. All 19S IgM, and, presence complement (C), had high lytic titers on T appropriate antigenicity. Their specificity was shown by lysis Thy-1 congenic strains, A/J(Thy-1.2) A.Thy 1.1. Furthermore, they lyse only 60 70% lymph node cells, suggesting cytotoxicity mature not B cells. Treatment peripheral lymphocyte populations monoclonal antibody plus C eliminated effector cytotoxic lymphocytes, their precursors, mitogenic response Con A, but did affect LPS. Purified, fluorescein-labeled anti-Thy-1 could be used distinguish Purified coupled Sepharose 6MB separate viable Two independently isolated anti-Thy-1.2 hybridomas are indistinguishable bind same determinant whereas a third is unique may site.