Detection of aflatoxin-producing molds in Korean fermented foods and grains by multiplex PCR.
作者： ZHENG-YOU YANG , WON-BO SHIM , JI-HUN KIM , SEON-JA PARK , SUNG-JO KANG
DOI: 10.4315/0362-028X-67.11.2622
关键词:
摘要: An assay based on multiplex PCR was applied for the detection of potential aflatoxin-producing molds in Korean fermented foods and grains. Three genes, ajfA, omtA, ver-I, coding key enzymes aflatoxin biosynthesis, were used as aflatoxin-detecting target genes PCR. DNA extracted from Aspergillus flavus, parasiticus, oryzae, niger, terreus, Penicillius expansum, Fusarium verticillioides template to test specificity assay. Positive results achieved only with that aflatoxigenic A. flavus parasiticus all three primer pairs. This result supported by direct competitive enzyme-linked immunosorbent (DC-ELISA). The required just a few hours, enabling rapid simultaneous many samples at low cost. A total 22 Meju samples, 24 Doenjang 10 barley commercially obtained Korea analyzed. DC-ELISA gave negative whereas PCR-based method positive 1 2 samples. After incubation malt extract agar, also detection. All assay, suggesting contamination presence are probably low.
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