Micro-characterization of modified microemulsions loaded with gossypol, pure and extracted from cottonseed.

摘要: Abstract Microemulsions (MEs) have gained increasing interest as carriers of hydrophobic bioactives in the last decades. However, it is still difficult to control uptake and release directly extracted from plants. In this study, modified ME nanodroplets (nano-sized self-assembled liquids, NSSLs) were employed extraction medium gossypol, a toxic component cottonseed. Loading was performed using both pure gossypol obtained by We achieved two goals: i) remove cottonseed obtain cotton-oil free gossypol; ii) extract into nano-delivery vehicle for biomedical purposes. Structural dynamical information on unloaded gossypol-loaded NSSL systems self-diffusion nuclear magnetic resonance, SD-NMR, spin-probe electron paramagnetic resonance (EPR) studies. The results showed that formed fluid water-in-oil (W/O) nano domains at lowest water contents; more viscous bicontinuous structure comparable oil contents, and, finally, oil-in-water (O/W, micellar-like) higher concentration water. These micellar-like structures external hydrated surface, demonstrated while lipidic region tested EPR revealed an packing. all these structures, mainly localized lipophilic close interface. Overall, SD-NMR provided complementary information, helping clarify structural properties NSSLs different contents their ability incorporate also cottonseed-NSSL mixtures.