Histologic localization of indocyanine green dye in aging primate and human ocular tissues with clinical angiographic correlation

摘要: Abstract Objective This study aimed to histologically localize indocyanine green (ICG) dye in the geriatric primate and human eye correlate these findings with clinical ICG angiography. Design The design was a clinicopathologic correlation. Participants Six eyes of three monkeys ( Maccaca mulatta ) macular drusen, 19 29 years age, housed at California Primate Research Center an enucleated from 66-year-old patient choroidal melanoma were examined. Intervention All six monkey underwent Five varying intervals after intravenous injection for histologic examination. One removed without prior as age-matched control. dye. Main outcome measures Infrared fluorescence microscopy freeze-dried tissue sections performed detect fluorescence. Histologic stimulated 810-nm diode laser, emitted detected Hamamatsu infrared camera. images digitally recorded. distribution on examination correlated angiogram. Results localized within retinal vessels early seen extravascular stroma 10 minutes injection. stromal persisted obtained 50 ICG. pigment epithelium (RPE)-Bruch's membrane complex brightly fluorescent middle- latestage sections. Drusen deposits all timepoints Similar observed eye. closely angiograms same interval. Conclusions does not remain solely intravascular space but extravasates into accumulates RPE. Extravascular binds drusen material. These will enhance interpretation