Thermal transitions in mixtures of polydeoxyribodinucleotides.
作者： F. N. Hayes , E. H. Lilly , R. L. Ratliff , D. A. Smith , D. L. Williams
DOI: 10.1002/BIP.1970.360090911
关键词:
摘要: Oligo d(C-A) and oligo d(T-G) of known average lengths, prepared by a combination chemical enzymatic procedures, have been mixed in 0.02 M 0.07 Na+, absorbance has studied as function increasing temperature. The transitions analyzed for the temperature maximum slope Tm, breadth transition, value at hyperchromicity. Linear expressions found relating inverse length nucleotide units (n−1) shorter oligomer, irrespective its identity, to Tm also transition breadth. From difference between versus n−1 two molarities, entropy enthalpy melting calculated n−1.
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