Human bone marrow stromal cell and ligament fibroblast responses on RGD‐modified silk fibers
作者： Jingsong Chen , Gregory H. Altman , Vassilis Karageorgiou , Rebecca Horan , Adam Collette
DOI: 10.1002/JBM.A.10120
关键词:
摘要: Adhesion, spreading, proliferation, and collagen matrix production of human bone marrow stromal cells (BMSCs) on an RGD-modified silk was studied. Anterior cruciate ligament fibroblasts (ACLFs) were used as a control cell source. Scanning electron microscopy (SEM) MTT analyses demonstrated that the modified matrices support improved BMSC ACLF attachment show higher density over 14 days in culture when compared with non-RGD-modified matrices. Collagen type I transcript levels (at day 7) content 14) significantly substrate than nonmodified group. The ability RGD-coupled to attachment, which leads vitro, combined mechanical, fatigue, biocompatibility properties protein matrix, suggest potential for use this biomaterial tissue engineering.
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