Genetically Encoded Fluorescent Probes and Live Cell Imaging

摘要: Genetically encoded fluorescent probes have revolutionized modern cell biology and physiology. Stemming from the initial discovery of crystal jelly’s green protein, these tools now permit researchers to study dynamic, intricate structures, molecular interactions in living cells. This article outlines many ingenious ways genetically proteins are used image cells, both petri dish organisms – how they can be conjunction with new technologies break diffraction barrier obtain ultrastructural information. The major technical challenges considerations for using outlined, along their derivatives construct biosensors that probe intracellular chemical environment.