Infectious cause of death determination using minimally invasive autopsies in developing countries

摘要: In developing countries, the knowledge of microorganisms causing fatal infections is critical and could help designing implementing more effective preventive interventions treatment guidelines. We aimed to develop validate protocols for microbiological analysis in post-mortem samples obtained during minimally invasive autopsy (MIA) procedures assess their performance. Thirty MIAs performed adults at Maputo Central Hospital Southern Mozambique were included analysis. Microbiological tests a universal screening HIV, hepatitis B C viruses, Plasmodium falciparum, bacterial/fungal culture. addition, variety molecular microbiology assays guided by histological results blood, cerebrospinal fluid tissue including liver, lung central nervous system. The combination culture-based methods together with led identification 17 out 19 (89.5%) infectious deaths. Microorganisms identified Mycobacterium tuberculosis, Toxoplasma gondii, Pneumocystis jiroveci, Cryptococcus neoformans, virus, human herpesvirus 8, cytomegalovirus, Streptococcus pneumoniae, dysgalactiae, Ryzopus oryzae, Acinetobacter baumannii. classical cultures, serological through MIA allows most agents death.