Profiles of Amyloid Precursor and Presenilin 2-Like Proteins are Correlated During Development of the Mouse Hypothalamus

摘要: The amyloid precursor protein (APP) and APP-like (APLP) material, as visualized with the Mab22C11 antibody, have previously been shown to be associated radial glia in hypothalamus, which are known promote neurite outgrowth. By Northern blot analysis, APP 695 mRNA levels increased steadily over hypothalamic development, 770 was transiently expressed at 12 days postnatally, APLP only weakly hypothalamus. developmental pattern of moeities mouse hypothalamus fetal neurons culture compared a presenilin 2 (PS2) related using an antibody developed against N-terminal part PS2. Western PS2-like immunoreactivity were 100-130 52 kDa bands, respectively. An biphasic increase observed during development vivo. equally detected neuronal glial cultures, while material more concentrated neurons. A correlation between APP/APP-like While mostly membrane fractions, significant portion also recovered from cytosolic fractions vitro. In contrast native PS2 COS-transfected cells, did not aggregate after heating for 90 s degrees C. These results indicate close association vivo, suggest that cultures may provide appropriate models test their interactions.