Activation of the MAPK11/12/13/14 (p38 MAPK) pathway regulates the transcription of autophagy genes in response to oxidative stress induced by a novel copper complex in HeLa cells.

摘要: Transition metal copper (Cu) can exist in oxidized or reduced states cells, leading to cytotoxicity cancer cells through oxidative stress. Recently, complexes are emerging as a new class of anticancer compounds. Here, we report that novel complex (HYF127c/Cu) induces stress-dependent cell death cells. Further, transcriptional analysis revealed stress elicits broad changes genes, which autophagy-related genes significantly changed HYF127c/Cu-treated Consistently, autophagy was induced and inhibitors promoted by HYF127c/Cu. Further identified the MAPK11/12/13/14 (formerly known p38 MAPK) pathway also activated Meanwhile, inhibitor SB203580 downregulated inhibiting transcription MAP1LC3B, BAG3, HSPA1A, HYF127c/Cu-induced death. These data suggest copper-induced will induce protective regulation activation HeLa