Isolation of Major Phenolic Compounds from the Extracts of Prunella L. Species Grown in Turkey and Their Antioxidant and Cytotoxic Activities
作者： Saliha Şahin , Ferda Ari , Cevdet Demir , Engin Ulukaya
DOI: 10.1111/JFBC.12043
关键词:
摘要: The major phenolics of Prunella L. species were isolated, and antioxidant activites determined by 2,2-azino-di-(3-ethylbenzothialozine-sulphonic acid). isolated compounds identified as rutin, rosmarinic acid caffeic high performance liquid chromatography-diode array detection. results show that the phenolic from with content ranged 74% (Prunella grandiflora L.) to 99% orientalis Bornm.), compound was above 90% in all species. Antigrowth effects tested against human breast cancer cell lines, MCF-7 (estrogen receptor-positive) MDAMB231 receptor-negative) MTT (3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazoliumbromide) ATP (adenosine triphosphate) viability assays. To determine mode death, M30-antigen assay performed order measure apoptosis. It found laciniata (L.) L., P. Bornm. showed antigrowth effect on MDA-MB-231 cells, but apoptosis not observed.
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sci-hub.se 参考文章(34)
Petitjean-Freytet C, Lamaison Jl, Carnat A, [Medicinal Lamiaceae with antioxidant properties, a potential source of rosmarinic acid]. Pharmaceutica Acta Helvetiae. ,vol. 66, pp. 185- 188 ,(1991)
J. L. Lamaison, C. Petitjean-Freytet, Hydroxycinnamic derivatives and flavonoids in the genus Prunella (Lamiaceae): antioxidant activities and chemotaxonomic value. Plantes Médicinales et Phytothérapie. ,vol. 24, pp. 152- 157 ,(1990)
Marian V. Eberhardt, Chang Yong Lee, Rui Hai Liu, Nutrition: Antioxidant activity of fresh apples Nature. ,vol. 405, pp. 903- 904 ,(2000) , 10.1038/35016151
Sang-Hoon Choi, Tae Bong Kang, Uiyoung Lee, Taek Joon Yoon, Yong-Sun Cho, Jong-Gun Won, Jong-Cheol Lim, Ae-Jung Kim, Jun Beom Lee, Hak-Cheon Lee, Chang-Jin Jeong, Effect of Prunella vulgaris Labiatae Extract on Innate Immune Cells and Anti-metastatic Effect in Mice Food Science and Biotechnology. ,vol. 18, pp. 218- 222 ,(2009)
Peter E Andreotti, Ian A Cree, Christian M Kurbacher, Diana M Hartmann, Dee Linder, Guy Harel, Ira Gleiberman, Phillip A Caruso, Sharon H Ricks, M Untch, Christian Sartori, Howard W Bruckner, Chemosensitivity Testing of Human Tumors Using a Microplate Adenosine Triphosphate Luminescence Assay: Clinical Correlation for Cisplatin Resistance of Ovarian Carcinoma Cancer Research. ,vol. 55, pp. 5276- 5282 ,(1995)
Jae Ho Choi, Eun Hee Han, Yong Pil Hwang, Jun Min Choi, Chul Yung Choi, Young Chul Chung, Jong Kwon Seo, Hye Gwang Jeong, Suppression of PMA-induced tumor cell invasion and metastasis by aqueous extract isolated from Prunella vulgaris via the inhibition of NF-κB-dependent MMP-9 expression Food and Chemical Toxicology. ,vol. 48, pp. 564- 571 ,(2010) , 10.1016/J.FCT.2009.11.033
Engin Ulukaya, Ferda Ozdikicioglu, Arzu Yilmaztepe Oral, Meral Demirci, None, The MTT assay yields a relatively lower result of growth inhibition than the ATP assay depending on the chemotherapeutic drugs tested Toxicology in Vitro. ,vol. 22, pp. 232- 239 ,(2008) , 10.1016/J.TIV.2007.08.006
M. Minnunni, U. Wolleb, O. Mueller, A. Pfeifer, H.U. Aeschbacher, Natural antioxidants as inhibitors of oxygen species induced mutagenicity Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. ,vol. 269, pp. 193- 200 ,(1992) , 10.1016/0027-5107(92)90200-L
A Zdařilová, A Svobodová, V Šimánek, J Ulrichová, None, Prunella vulgaris extract and rosmarinic acid suppress lipopolysaccharide-induced alteration in human gingival fibroblasts Toxicology in Vitro. ,vol. 23, pp. 386- 392 ,(2009) , 10.1016/J.TIV.2008.12.021
D. Baricevic, S. Sosa, R. Della Loggia, A. Tubaro, B. Simonovska, A. Krasna, A. Zupancic, Topical anti-inflammatory activity of Salvia officinalis L. leaves: the relevance of ursolic acid. Journal of Ethnopharmacology. ,vol. 75, pp. 125- 132 ,(2001) , 10.1016/S0378-8741(00)00396-2