Genetic Tools for Use with Listeria monocytogenes

摘要: The number and sophistication of genetic tools that have become available in recent years for the molecular characterization Listeria monocytogenes continued to increase. Plasmid vectors, reporter genes, systems designed transposon mutagenesis, heterologous expression systems, integration transducing phage all greatly advanced experimental capacity generate, characterize, complement mutations within L. define functional roles gene products. This chapter provides a brief description currently use with monocytogenes. Key references are given throughout this provide sources expanded details on plasmid constructions, assay conditions, other technical aspects. variety described is meant be representative resources those interested genetics. More widely used studying profiles bacteria whole-genome DNA macro- microarrays, which comprehensive transcriptional analysis enabling researchers view organism as system. Several genes developed proven useful monitoring regulation. Transcriptional fusions such lacZ, gus, gfp, lux, cat been constructed successfully monitor patterns bacterial culture infected cells animals. advantages and/or disadvantages some these discussed briefly.