Gold Nanoparticles Prepared with Phyllanthus emblica Fruit Extract and Bifidobacterium animalis subsp. lactis Can Induce Apoptosis via Mitochondrial Impairment with Inhibition of Autophagy in the Human Gastric Carcinoma Cell Line AGS.

作者: Hoon Kim , Yeon-Ju Kim , Haribalan Perumalsamy , Sri Renukadevi Balusamy , Aditi Mitra Puja

DOI: 10.3390/NANO11051260

关键词:

摘要: (1) Background: Nanotechnology is being widely applied for anticancer strategies with few side effects. Nanoparticles (NPs) prepared from natural extracts are promising candidates cancer treatment because of their unique physicochemical characteristics. This study aimed to prepare gold nanoparticles (AuNPs) Phyllanthus emblica fruit extract (PEFE) using Bifidobacterium animalis subsp. lactis (B. lactis) and evaluate activity against the human gastric adenocarcinoma cell-line (AGS). (2) Methods: The safety microbial biosynthesis AuNPs (PEFE-AuNPs) was assessed by evaluating cytotoxicity. PEFE-AuNPs investigated in AGS cells terms apoptosis autophagy. (3) Results: exhibited significant cytotoxicity but not normal cells. induced associated PTEN-induced kinase 1 (PINK1)-Parkin mediated reduction mitochondrial membrane potential activation intracellular signaling pathways. induction through inhibition autophagy, downregulation LC3-II/LC3-I Beclin-1 expression, upregulation p62 expression (4) Conclusions: first demonstrate Our results provide a good starting point development new products based on P. extract.

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