Regeneration competence accompanies increased expression of arginine methyltransferase PRMT8 in human adult fibroblasts

摘要: Identification of therapeutically relevant molecules is necessary for the advancement non-viral reprogramming human cells regenerative medicine. We have developed a novel model system that transforms primary dermal fibroblasts into with induced regeneration competence (iRC). Low oxygen-mediated effects fibroblast growth factor FGF2 lead to an increased cellular lifespan two fold increase in population doublings before senescence, remaining non-tumorigenic when injected SCID mice while maintaining [1, 2]. This allows us study participate system. project aims identify unique contribute iRC phenotype goal design therapeutics target diseases associated aging, wound healing, and tumor formation. Analysis 84 chromatin modification enzymes by qRT-PCR revealed 13-fold upregulation arginine methyltransferase PRMT8 cells. Increased protein expression was confirmed both embryonic stem - first demonstration endogenous expression. These results may ultimately findings regarding regulation methyltransferases define functions