Analysis of Ia induction on Lewis rat astrocytes in vitro by virus particles and bacterial adjuvants.

摘要: Abstract Viral particles of a neurotropic murine hepatitis virus (JHM) and various substances known to have immunoregulatory effects, including bacterial lipopolysaccharide (LPS) synthetic adjuvant peptide (muramyl dipeptide) (AP), were tested for their ability induce Ia antigen expression on Lewis rat astrocytes in vitro. JHM virus, LPS AP are all capable inducing molecules astrocytes, however, pattern kinetics distinct from recombinant gamma interferon (γ-IFN). Whereas γ-IFN induced macrophages after 48 h treatment, required 4–7 days maximal induction but had little no effect the macrophage population. This indicates that uniquely reactive components derived infectious agents these with respect astrocytes. We also attempted determine possible mechanisms by which astrocyte show phorbol myristate acetate Ca2+ ionophore A23187 similar effects. These findings suggest may directly stimulate presenting functions brain through γ-IFN-independent mechanisms.