Cymbidium mosaic potexvirus RNA: complete nucleotide sequence and phylogenetic analysis.

作者: S. M. Wong , P. H. Mahtani , K. C. Lee , H. H. Yu , Y. Tan

DOI: 10.1007/S007050050084

关键词:

摘要: The complete nucleotide sequence of the genomic RNA cymbidium mosaic potexvirus (CymMV) was determined to be 6 227 nucleotides in length, excluding poly (A) tail at 3′ terminus. Similar other potexviruses, its genome organisation is comprised five major open reading frames (ORFs 1 5), encoding a Mr 160 KDa putative RNA-dependent polymerase (RdRp); Mr26KDa/13KDa/10KDa triple-gene-block (TGB) and 24 KDa coat protein. CymMV encoded proteins shared high degree homology their corresponding members group. 5′ noncoding region (NCR) all potexviruses initiates with GAAAA. possesses shortest NCR among potexviruses. Based on phylogenetic comparisons RdRp protein, shares close relationship PAMV, NMV, WClMV SMYEaV. This believed first record CymMV.

参考文章(25)
D. D. Jensen, Mosaic or black streak disease of Cymbidium Orchids. Phytopathology. ,vol. 41, ,(1951)
Koh-Koh Neo, Sek-Man Wong, Mian Wu, Nucleotide sequence and in vitro translation of the coat protein gene of cymbidium mosaic virus. Virus Genes. ,vol. 7, pp. 157- 170 ,(1993) , 10.1007/BF01702396
K.Andrew white, J.B. Bancroft, George A. Mackie, Mutagenesis of a hexanucleotide sequence conserved in potexvirus RNAs. Virology. ,vol. 189, pp. 817- 820 ,(1992) , 10.1016/0042-6822(92)90614-U
S.M. Wong, C.G. Chng, Y.H. Lee, K. Tan, F.W. Zettler, Incidence of cymbidium mosaic and odontoglossum ringspot viruses and their significance in orchid cultivation in Singapore. Crop Protection. ,vol. 13, pp. 235- 239 ,(1994) , 10.1016/0261-2194(94)90084-1
K. G. Skryabin, A. S. Kraev, S. Yu. Morozov, M. N. Rozanov, B. K. Chernov, L. I. Lukasheva, J. G. Atabekov, The nucleotide sequence of potato virus X RNA. Nucleic Acids Research. ,vol. 16, pp. 10929- 10930 ,(1988) , 10.1093/NAR/16.22.10929
K.G. Skryabin, S.Yu. Morozov, A.S. Kraev, M.N. Rozanov, B.K. Chernov, L.I. Lukasheva, J.G. Atabekov, Conserved and variable elements in RNA genomes of potexviruses FEBS Letters. ,vol. 240, pp. 33- 40 ,(1988) , 10.1016/0014-5793(88)80335-1
Richard L.S. Forster, Michael W. Bevan, Sally-Ann Harbison, Richard C. Gardner, The complete nucleotide sequence of the potexvirus white clover mosaic virus. Nucleic Acids Research. ,vol. 16, pp. 291- 303 ,(1988) , 10.1093/NAR/16.1.291
Eugene V. Koonin, Valerian V. Dolja, T. Jack Morris, Evolution and Taxonomy of Positive-Strand RNA Viruses: Implications of Comparative Analysis of Amino Acid Sequences Critical Reviews in Biochemistry and Molecular Biology. ,vol. 28, pp. 375- 430 ,(1993) , 10.3109/10409239309078440