Extraction of thyrotrophin from pituitary glands, mouse pituitary tumors, and blood plasma by percolation.

摘要: A percolation method of extraction thyrotrophin from dry powders anterior pituitary glands, mouse tumors and blood plasma has been developed. The powder mixed with an equal weight filter aid suspended in 95% ethanol was placed on a Buchner funnel increasingly more aqueous solutions containing salt were run succession through the cake stepwise manner. TSH is removed 57 or 67% saturated sodium chloride. yields estimated to be over 50% potency about 2% weight, which concentration activity 30 times, whether dried powder.