The investigation of toluidine blue and loss of heterozygosity patterns in oral premalignant lesions

摘要: Individuals with oral squamous cell carcinomas (SCCs) have a generally poor prognosis mainly due to late diagnosis of the disease. The identification early stages disease (oral premalignant lesions, OPLs) is crucial improving outcome. At present, prediction outcome for OPLs relies on histological assessment biopsies presence and degree dysplasia. Unfortunately, pathology predictor earliest, most frequently observed OPLs. Our recent study has shown that high-risk can be identified by assessing loss heterozygosity (LOH) using microsatellite analysis. both molecular assessments depend availability accuracy biopsy. determination when where biopsy continuing challenge facing clinicians. In experienced hands, toluidine blue (TB) staining used facilitate such determinations. However, at present it not clear whether TB-positive differ in alterations cancer risk from negative lesions. objective this thesis was determine LOH profiles were different TB positive A total 67 evaluated staining, assessed 11 markers chromosome arms: 3p, 9p, 17p. main finding cases had than cases. They showed increased losses multiple regions (P = 0.004), 3p 0.04), 9p =0.007), 17p 0.0001). This association between independent hyperplasia or low-grade dysplasia higher frequencies any 0.006) 0.005). conclusion, these data show patterns allelic are TB-negative lesions an proportion showing elevated progression. support use dye clinicians identify tissue requires