Artifact-free, penetration-adjustable elliptical-mirror-based TIRF microscopy

作者: Jian Liu , Chenqi Kong , Qiang Li , Weisong Zhao , Mengzhou Li

DOI: 10.1364/OE.26.026065

关键词:

摘要: Evanescent field distribution in the focal region of elliptical-mirror-based total-internal-reflection fluorescence (e-TIRF) microscopy is analyzed based on vectorial diffraction theory. The simulation demonstrates that intensity an evanescent generated by elliptical mirror decreases exponentially with penetration depth, and polarization characteristic wave various directions given. We build up e-TIRF microscope utilizing a focused hollow-cone illumination all-direction large range incidence. experiment shows artifact effect can be well suppressed using azimuthal-direction method. In addition, depth controlled adjusting sizes aperture obstruction range.

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