Vesicle and bilayer formation of diphytanoylphosphatidylcholine (DPhPC) and diphytanoylphosphatidylethanolamine (DPhPE) mixtures and their bilayers’ electrical stability

摘要: Lipid bilayers are of interest in applications where a cell membrane mimicking environment is desired. The performance the lipid bilayer largely dependent on physical and chemical properties component lipids. consisting phytanoyl lipids have proven to be appropriate choices since they exhibit high mechanical stability. In addition, such electrical resistances. Two different lipids, 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC) 1,2-diphytanoyl-sn-glycero-3-phosphoethanolamine(DPhPE), various combinations two been investigated with respect their behavior aqueous solutions, interactions solid surfaces, Dynamic light scattering, nuclear magnetic resonance diffusion, cryogenic transmission electron microscopy measurements showed that pure DPhPC as well mixtures DPhPE greater than 50% (mol%) formed unilamellar vesicles. If total concentration was 0.15 g/l, then vesicles solid-supported plasma-treated gold silica surfaces by process spontaneous vesicle adsorption rupture, determined quartz crystal microbalance dissipation monitoring atomic force microscopy. exhibited degree viscoelasticity, probably an effect relatively amounts imbibed water or incomplete fusion. compositions small flower-like vesicular structures along discrete liquid crystalline structures, evidenced Furthermore, electrophysiology were performed using tip-dip methodology bilayers' capacity retain its resistance towards applied potential across evaluated function composition. It shown ratio significantly affected bilayer's stability, having highest stability followed 3DPhPC:7DPhPE 7DPhPC:3DPhPE decreasing order. 5DPhPC:5DPhPE had lowest potential.