Amino acid sequence of small and large subunits of seed storage protein from Ricinus communis.
作者： F S Sharief , S S Li
DOI: 10.1016/S0021-9258(18)33344-1
关键词:
摘要: The low molecular weight, glutamine-rich storage protein isolated from the seeds of Ricinus communis (castor beans) has been shown to consist two different polypeptide chains linked by disulfide bond(s). small subunit is composed 34 amino acids with a proline at its NH2 terminus, whereas large contains 61 cyclized glutamine as NH2-terminal residue. complete acid sequence both subunits determined through characterization and selected peptides trypsin, chymotrypsin, thermolysin, cyanogen bromide cleavage. intact possesses number glutaminyl half-cystinyl residues exhibits heterogeneity observed peptide sequences. Comparison this those other seed proteins indicates some structural similarities between them. sequences castor bean are: (formula, see text).
sci-hub.st 参考文章(37)
C.H.W. Hirs, [19] Performic acid oxidation Methods in Enzymology. ,vol. 11, pp. 197- 199 ,(1967) , 10.1016/S0076-6879(67)11021-5
D.G. Smyth, [24] Techniques in enzymic hydrolysis Methods in Enzymology. ,vol. 11, pp. 214- 231 ,(1967) , 10.1016/S0076-6879(67)11026-4
R.E. Offord, [6] The use of logarithmic plots of electrophoretic mobilities of peptides Methods in Enzymology. ,vol. 47, pp. 51- 69 ,(1977) , 10.1016/0076-6879(77)47008-3
Kenneth G Mann, Wayne W Fish, [2] Protein polypeptide chain molecular weights by gel chromatography in guanidinium chloride Methods in Enzymology. ,vol. 26, pp. 28- 42 ,(1972) , 10.1016/S0076-6879(72)26004-9
Robert L. Heinrikson, [20] Applications of thermolysin in protein structural analysis Methods in Enzymology. ,vol. 47, pp. 175- 189 ,(1977) , 10.1016/0076-6879(77)47022-8
Celine G. L. Tan, Frits C. Stevens, Amino acid sequence of lima bean protease inhibitor component IV. 2. Isolation and sequence determination of the chymotryptic peptides and the complete amino acid sequence. FEBS Journal. ,vol. 18, pp. 515- 523 ,(1971) , 10.1111/J.1432-1033.1971.TB01271.X
T C Vanaman, S J Wakil, R L Hill, The preparation of tryptic, peptic, thermolysin, and cyanogen bromide peptides from the acyl carrier protein of Escherichia coli. Journal of Biological Chemistry. ,vol. 243, pp. 6409- 6419 ,(1968) , 10.1016/S0021-9258(18)93154-6
M.A. Moreira, M.A. Hermodson, B.A. Larkins, N.C. Nielsen, Partial characterization of the acidic and basic polypeptides of glycinin. Journal of Biological Chemistry. ,vol. 254, pp. 9921- 9926 ,(1979) , 10.1016/S0021-9258(19)83605-0
Bo Lönnerdal, Jan-Christer Janson, Studies on Brassica seed proteins. I. The low molecular weight proteins in rapeseed. Isolation and characterization. Biochimica et Biophysica Acta. ,vol. 278, pp. 175- 183 ,(1972) , 10.1016/0005-2795(72)90119-5
D.M. Watterson, F. Sharief, T.C. Vanaman, The complete amino acid sequence of the Ca2+-dependent modulator protein (calmodulin) of bovine brain. Journal of Biological Chemistry. ,vol. 255, pp. 962- 975 ,(1980) , 10.1016/S0021-9258(19)86127-6