Fabrication of novel hepatoma-targeting microdisks by hydrogen bond-assisted self-assembly of an azacitidine-conjugating amphiphilic random copolymer
作者： Xia Li , Hui Guo , Junliang Wang , Qi Wu , Xianfu Lin
DOI: 10.1016/J.ACTBIO.2009.07.002
关键词:
摘要: Novel hepatoma-targeting azacitidine-conjugating microdisks were successfully fabricated by hydrogen bond-assisted self-assembly of an amphiphilic random copolymer with galactose and azacitidine as pendants, poly(5'-O-vinyladipyl-azacitidine-co-6-O-vinylsebacyl-galactose). The was easily prepared a two-step chemoenzymatic synthetic route, the possibility its verified ultraviolet-visible fluorescence spectroscopy using pyrene hydrophobic probe. Transmission electron microscopy scanning indicated that aggregation morphologies these self-assemblies thin disks radii from 250 to 500nm. Section analysis atomic force for disk gave thickness approximately 16nm. existence bonds among pendants investigated infrared spectra Gaussian calculation. Cellular uptake assay observed confocal laser cell cytotoxicity tests MTT dye reduction method showed had evident function hepG2 human hepatoma cells could effectively realize cellular internalization azacitidine.
elsevier.com
sci-hub.se 参考文章(35)
Fwu-Long Mi, Yong-Yi Wu, Ya-Lin Chiu, Mei-Chin Chen, Hsing-Wen Sung, Shu-Huei Yu, Shin-Shing Shyu, Mei-Feng Huang, Synthesis of a novel glycoconjugated chitosan and preparation of its derived nanoparticles for targeting HepG2 cells. Biomacromolecules. ,vol. 8, pp. 892- 898 ,(2007) , 10.1021/BM060998B
Michelle A. Rudek, Ming Zhao, Ping He, Carol Hartke, Jill Gilbert, Steven D. Gore, Michael A. Carducci, Sharyn D. Baker, Pharmacokinetics of 5-Azacitidine Administered With Phenylbutyrate in Patients With Refractory Solid Tumors or Hematologic Malignancies Journal of Clinical Oncology. ,vol. 23, pp. 3906- 3911 ,(2005) , 10.1200/JCO.2005.07.450
Jintao Zhu, Haizhou Yu, Wei Jiang, Morphological Transition of Aggregates from ABA Amphiphilic Triblock Copolymer Induced by Hydrogen Bonding Macromolecules. ,vol. 38, pp. 7492- 7501 ,(2005) , 10.1021/MA0510744
K. Kalyanasundaram, J. K. Thomas, Environmental effects on vibronic band intensities in pyrene monomer fluorescence and their application in studies of micellar systems Journal of the American Chemical Society. ,vol. 99, pp. 2039- 2044 ,(1977) , 10.1021/JA00449A004
William F. Edmonds, Zhibo Li, Marc A. Hillmyer, Timothy P. Lodge, Disk Micelles from Nonionic Coil−Coil Diblock Copolymers Macromolecules. ,vol. 39, pp. 4526- 4530 ,(2006) , 10.1021/MA060633J
Dao Cong Dong, Mitchell A. Winnik, The Py scale of solvent polarities Canadian Journal of Chemistry. ,vol. 62, pp. 2560- 2565 ,(1984) , 10.1139/V84-437
Bing Shi Li, Kevin K. L. Cheuk, Deliang Yang, Jacky W. Y. Lam, Li Jun Wan, Chunli Bai, Ben Zhong Tang, Self-Assembling of an Amphiphilic Polyacetylene Carrying l-Leucine Pendants: A Homopolymer Case Macromolecules. ,vol. 36, pp. 5447- 5450 ,(2003) , 10.1021/MA034275L
Faysal Ilhan, Trent H. Galow, Mark Gray, Gilles Clavier, Vincent M. Rotello, Giant Vesicle Formation through Self-Assembly of Complementary Random Copolymers Journal of the American Chemical Society. ,vol. 122, pp. 5895- 5896 ,(2000) , 10.1021/JA0011966
Q. Yuan, R. Venkatasubramanian, S. Hein, R.D.K. Misra, A stimulus-responsive magnetic nanoparticle drug carrier: magnetite encapsulated by chitosan-grafted-copolymer. Acta Biomaterialia. ,vol. 4, pp. 1024- 1037 ,(2008) , 10.1016/J.ACTBIO.2008.02.002
Maria Antonietta Casadei, Giovanna Pitarresi, Rossella Calabrese, Patrizia Paolicelli, Gaetano Giammona, Biodegradable and pH-sensitive hydrogels for potential colon-specific drug delivery: characterization and in vitro release studies. Biomacromolecules. ,vol. 9, pp. 43- 49 ,(2008) , 10.1021/BM700716C