作者: Daniela C. Sabaté , Carolina Pérez Brandan , Gabriela Petroselli , Rosa Erra-Balsells , M.Carina Audisio
DOI: 10.1016/J.MICRES.2018.04.003
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摘要: Abstract Bacillus sp. B19, P12 and B. amyloliquefaciens B14 were isolated from soils of Salta province, PGPR properties on the common bean (Phaseolus vulgaris L.) cv. Alubia antagonistic activity against Sclerotinia sclerotiorum studied. It was determined that B19 increased crop germination potential (GP) by 14.5% compared to control seeds; these strains also root length (10.4 15%, respectively) stem (20.2 30%, control; however, as for strain, no increases in growth parameters detected. In addition, all treatments combined two bacilli: B14 + B19, B14 + P12 B19 + P12, generated beneficial effects GP seedling seeds, but not a single inoculant. synthesized auxins at concentrations 5.71 4.90 mg/mL, respectively, it qualitatively they synthesize siderophores. previous studies have produces concentration 10.10 mg/mL, synthesizes The phytosanitary state white seeds revealed bacterial contamination 87% evaluated different fungi such Cladosporium sp., Fusarium Rhizopus Bean treated with B14, or showed contaminating bacteria pathogenic fungi; fact, bacilli inoculum development observed seeds. Additionally, inhibited vitro 9 native S. region, FI ranging between 60 100%. three isoforms lipopeptides: surfactin, iturin, fengycin presence sclerotiorum, MALDI-TOF. vivo trials, when grown contaminated an incidence 100% any Bacillus. Seeds chemical fungicide sown sclerotiorum-infested soil did produce seed emergence, while inoculation B14 + P12, B14 + B19 B19 + P12 reduced effect pathogen 46, 43 25%, respectively. Disease progression B14 + P12 significantly lower than remaining treatments, AUDPC 873.75 1071,