A synthetic peptide substrate for selective assay of protein kinase C
作者： Ichiro Yasuda , Akira Kishimoto , Shin-ichiro Tanaka , Masahiro Tominaga , Atsushi Sakurai
DOI: 10.1016/0006-291X(90)90996-Z
关键词:
摘要: Abstract Among various phosphate acceptor proteins and peptides so far tested, a synthetic peptide having the sequence surrounding Ser (8) of myelin basic protein, Gln-Lys-Arg-Pro-Ser(8)-Gln-Arg-Ser-Lys-Tyr-Leu, (MBP 4–14 ), is most specific convenient substrate which can be used for selective assay protein kinase C. This not phosphorylated by cyclic AMP-dependent kinase, casein kinases I II, Ca 2+ /calmodulin-dependent or phosphorylase routinely C with low background in crude tissue extracts. The Km value considerably (7 μ M ) V max twice as much that Hl histone.
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