Physical Biochemistry : Principles and Applications

摘要: Preface. Chapter 1 Introduction. 1.1 Special Chemical Requirements of Biomolecules. 1.2 Factors Affecting Analyte Structure and Stability. 1.3 Buffering Systems Used in Biochemistry. 1.4 Quantitation, Units Data Handling. 1.5 The Worldwide Web as a Resource Physical 1.6 Objectives this Volume. References. 2 Chromatography. 2.1 Principles 2.2 Performance Parameters 2.3 Chromatography Equipment. 2.4 Modes 2.5 Open Column 2.6 High Liquid (HPLC). 2.7 Fast Protein 2.8 Perfusion 2.9 Membrane-Based Systems. 2.10 Sample Protein. 3 Spectroscopic Techniques. 3.1 Nature Light. 3.2 Electromagnetic Spectrum. 3.3 Ultraviolet/Visible Absorption Spectroscopy. 3.4 Fluorescence 3.5 Techniques Using Plane-Polarized 3.6 Infrared 3.7 Nuclear Magnetic Resonance (NMR) 3.8 Electron Spin (ESR) 3.9 Lasers. 3.10 Surface Plasmon Resonance. 4 Mass Spectrometry. 4.1 4.2 Spectrometry Proteins/Peptides. 4.3 Interfacing MS with other Methods. 4.4 Uses 5 Electrophoresis. 5.1 5.2 Nondenaturing 5.3 Denaturing 5.4 Electrophoresis DNA Sequencing. 5.5 Isoelectric Focusing (IEF). 5.6 Immunoelectrophoresis. 5.7 Agarose Gel Nucleic Acids. 5.8 Pulsed Field 5.9 Capillary 5.10 Electroblotting Procedures. 5.11 Electroporation. 6 Three-Dimensional Determination Macromolecules. 6.1 Protein-Folding Problem. 6.2 by NMR. 6.3 Crystallization Biomacromolecules. 6.4 X-Ray Diffraction Crystals. 6.5 Calculation Density Maps. 6.6 Other 6.7 Comparison Crystallography Multi-Dimensional 6.8 Structural Databases. 7 Hydrodynamic 7.1 Viscosity. 7.2 Sedimentation. 7.3 Methods for Varying Buffer Conditions. 7.4 Flow Cytometry. 8 Biocalorimetry. 8.1 Main Thermodynamic Parameters. 8.2 Isothermal Titration Calorimetry. 8.3 Differential Scanning 8.4 Non-Calorimetric Means. 9 Bioinformatics. 9.1 Overview 9.2 Sequence 9.3 Tools Analysis Primary Structures. 9.4 Tertiary 9.5 Programs Visualization 9.6 Homology Modelling. 10 Proteomics. 10.1 10.2 10.3 Chip Technologies 10.4 Post-Translational Modification Further Reading. Appendix SI Units. Fourier Transform. Index.