Novel polymeric bionanocomposites with catalytic Pt nanoparticles label immobilized for high performance amperometric immunoassay
作者： Yingchun Fu , Penghao Li , Ting Wang , Lijuan Bu , Qingji Xie
DOI: 10.1016/J.BIOS.2009.12.010
关键词:
摘要: Abstract We report on the aqueous preparation of novel polymeric bionanocomposites (PBNCs) with polydopamine (PDA) as an efficient matrix to support antibody and uniformly dispersed abundant Pt nanoparticles (PtNPs) for high performance sandwich-type amperometric immunoassay. prepare three kinds PBNCs (PBNCs 1 , 2 3 ) via chemical polymerization synthesis ( further adsorption or glutaldehyde (GA) covalent crosslinking antibody. Transmission electron microscopy, atomic force quartz crystal microbalance are used characterize PBNCs. The results show that PDA is excellent PtNPs due its biocompatibility, adsorbability, processibility, thus-prepared immuno-recognition efficiency catalytic activity toward H O reduction. Then, we examine applicability prepared immunosensing using a model immuno-pair human immunoglobulin G (hIgG) anti-hIgG, obtain signal by detecting electro-reduction catalyzed label. constructed immunosensors exhibit detection limits 0.068, 0.037, 0.018 ng mL −1 respectively, being competitive better than reported analogues. Our also present good reproducibility, stability, regeneration ability, specificity, satisfactory feasibility target assay in clinical serum samples. suggested protocol signal-transduction ability expected find wide bioassay applications.
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sci-hub.se 参考文章(54)
Yongchao Zhang, Adam Heller, Reduction of the nonspecific binding of a target antibody and of its enzyme-labeled detection probe enabling electrochemical immunoassay of an antibody through the 7 pg/ml-100 ng/mL (40 fM-400 pM) range. Analytical Chemistry. ,vol. 77, pp. 7758- 7762 ,(2005) , 10.1021/AC051218C
X MAO, J JIANG, Y LUO, G SHEN, R YU, Copper-enhanced gold nanoparticle tags for electrochemical stripping detection of human IgG. Talanta. ,vol. 73, pp. 420- 424 ,(2007) , 10.1016/J.TALANTA.2007.04.004
Jwa-Min Nam, C Shad Thaxton, Chad A Mirkin, Nanoparticle-based bio-bar codes for the ultrasensitive detection of proteins. Science. ,vol. 301, pp. 1884- 1886 ,(2003) , 10.1126/SCIENCE.1088755
Yingchun Fu, Qingji Xie, Xue’en Jia, Xiahong Xu, Wenhua Meng, Shouzhuo Yao, Electrosynthesized poly(1,6-hexanedithiol) as a new immobilization matrix for Au-nanoparticles-enhanced piezoelectric immunosensing Journal of Electroanalytical Chemistry. ,vol. 603, pp. 96- 106 ,(2007) , 10.1016/J.JELECHEM.2007.01.016
Guodong Liu, Jun Wang, Hong Wu, Yuehe Lin, None, Versatile apoferritin nanoparticle labels for assay of protein Analytical Chemistry. ,vol. 78, pp. 7417- 7423 ,(2006) , 10.1021/AC060653J
Xin Yu, Bernard Munge, Vyomesh Patel, Gary Jensen, Ashwin Bhirde, Joseph D Gong, Sang N Kim, John Gillespie, J Silvio Gutkind, Fotios Papadimitrakopoulos, James F Rusling, None, Carbon nanotube amplification strategies for highly sensitive immunodetection of cancer biomarkers. Journal of the American Chemical Society. ,vol. 128, pp. 11199- 11205 ,(2006) , 10.1021/JA062117E
Jiong Zhang, Shiping Song, Lihua Wang, Dun Pan, Chunhai Fan, A gold nanoparticle-based chronocoulometric DNA sensor for amplified detection of DNA Nature Protocols. ,vol. 2, pp. 2888- 2895 ,(2007) , 10.1038/NPROT.2007.419
Dianping Tang, Ruo Yuan, Yaqin Chai, Ultrasensitive electrochemical immunosensor for clinical immunoassay using thionine-doped magnetic gold nanospheres as labels and horseradish peroxidase as enhancer. Analytical Chemistry. ,vol. 80, pp. 1582- 1588 ,(2008) , 10.1021/AC702217M
Jagotamoy Das, Md. Abdul Aziz, Haesik Yang, A nanocatalyst-based assay for proteins: DNA-free ultrasensitive electrochemical detection using catalytic reduction of p-nitrophenol by gold-nanoparticle labels. Journal of the American Chemical Society. ,vol. 128, pp. 16022- 16023 ,(2006) , 10.1021/JA0672167
Prashanth Asuri, Sandeep S. Karajanagi, Ravi S. Kane, Jonathan S. Dordick, Polymer–Nanotube–Enzyme Composites as Active Antifouling Films† Small. ,vol. 3, pp. 50- 53 ,(2007) , 10.1002/SMLL.200600312